生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
不包含
preservative
种属反应性
rat, mouse, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer15)
基因信息
human ... TP53(7157)
一般描述
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
应用
Immunoblotting (1:1000)
Immunoprecipitation (1:200)
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
重悬
Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
分析说明
Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
其他说明
Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
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