MABT1475

Anti-Cytokeratin-8 Antibody, clone 1E8

• 别名: CYK8, KRT8, Keratin, type II cytoskeletal 8, Keratin-8 (K8), Type-II keratin Kb8
• 分類程式碼代碼: 12352203

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1E8, monoclonal
• 分子量: calculated mol wt 53.7 kDa; observed mol wt ~54 kDa
• 純化經由: using protein G
• 物種活性: human
• 物種活性（以同源性預測）: canine, monkey
• 包裝: antibody small pack of 100
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG2aκ
• 表位序列: C-terminal
• Protein ID登錄號: NP_001243222
• UniProt登錄號: P05787
• 儲存溫度: -10 to -25°C
• 基因資訊: human ... KRT8(3856)

说明

特異性

Clone 1E8 is a mouse monoclonal antibody that detects human Cytokeratin-8. It targets an epitope within 12 amino acids from the C-terminal part.

免疫原

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal of human Cytokeratin-8.

應用

Quality Control Testing

Evaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cytokeratin-8 in HaCaT cell lysate.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Cytokeratin-8 in Immunohistochemistry application (Sfakianos, J.P., et al. (2020). Nat Commun. 11(1):2540).

Western Blotting Analysis: A representative lot detected Cytokeratin-8 in Western Blotting application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Immunocytochemistry Analysis: A representative lot detected Cytokeratin-8 in Immunocytochemistry application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Inhibition Assay: An Anti-Cytokeratin-8, clone 1E8 Fab decreased plasminogen binding to breast cancer cell lines in Inhibition Assay (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. They share a common structural organization: a central -helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. It is a type II, neutral to basic protein that together with cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is highly conserved in all type II keratins. Phosphorylation at serine 73 is reported to increase during cellular stress. However, under normal conditions serine 73 remains largely dephosphorylated. It can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.

外觀

Purified mouse monoclonal antibody IgG2a in PBS without azide.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable