MABS2297

Anti-TPO Antibody, clone 47

• 别名: MoAb47, Thyroid peroxidase
• UNSPSC代码: 12352203
• NACRES: NA.43

属性

• 生物来源: mouse
• 质量水平: 200
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: 47, monoclonal
• 分子量: calculated mol wt 102.96 kDa; observed mol wt ~120 kDa
• 纯化方式: using protein G
• 种属反应性: human
• 包装: antibody small pack of 100
• 技术: ELISA: suitable; radioimmunoassay: suitable; western blot: suitable
• 同位素/亚型: IgG1κ
• 表位序列: Unknown
• Protein ID登记号: NP_000538
• UniProt登记号: P07202
• 储存温度: 2-8°C
• 基因信息: human ... TPO(7173)

说明

特异性

Clone 47 is a mouse monoclonal antibody that specifically detects Thyroid peroxidase.

免疫原

Human thyroid peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.

应用

Quality Control Testing

Evaluated by Western Blotting in human thyroid tissue extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Thyroid peroxidase (TPO) in human thyroid tissue extract.

Tested Applications

ELISA Analysis: A representative lot detected Thyroid peroxidase (TPO) in ELISA applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Radioimmunoassay: A representative lot detected Thyroid peroxidase (TPO) in Radioimmunoassay applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Thyroid peroxidase (Uniprot: P07202; also known as TPO) is encoded by gene TPO (Gene ID: 7173) in human. It is a single-pass type I membrane protein and has an Extracellular domain (19-846 aa), a Transmembrane domain ( 847-871 aa) and a Cytoplasmic domain (872-933 aa). It is primarily found in the thyroid gland and functions in the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine (T3 and T4). TPO is an integral part of the complex process by which the thyroid gland regulates metabolism and other vital bodily functions. Defects in this gene can cause congenital hypothyroidism, congenital goitre and thyroid hormone organification defect IIA. Moreover, TPO is a common target of autoimmune attacks in various thyroid disorders, such as Hashimoto′s thyroiditis and Graves′ disease, which can lead to disruptions in hormone synthesis and subsequent health issues.
(Ref.: Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8. (PMID: 2474433).

外形

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重悬

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommended storage: +2°C to +8°C.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable