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生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3F6, monoclonal
分子量
calculated mol wt 120.55 kDa
observed mol wt ~75 kDa
純化經由
using protein G
物種活性
monkey, rat, human, mouse
包裝
antibody small pack of 100 μL
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
表位序列
Internal
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... RAPGEF1(2889)
一般說明
Rap guanine nucleotide exchange factor 1 (UniProt: Q13905; also known as CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G) is encoded by the RAPGEF1 (also known as GRF2) gene (Gene ID: 2889) in human. RAPGEF1 is a ubiquitously expressed protein that regulates multiple cellular activities and is shown to be essential for early embryonic development in mammals. It is involved in cell proliferation, transformation, cytoskeletal organization, and apoptosis. It is induced during skeletal muscle cell and neuronal cell differentiation. Higher expression of RAPGEF1 is observed in adult skeletal muscle and placenta and in fetal brain and heart. Its phosphorylation at tyrosine 504 enhances its activity as Rap guanine nucleotide exchange factor. It has functions that depend on its catalytic activity as well as protein interaction domain. It contains four SH3 binding motifs (aa 281-292; 451-462; 538-549; and 606-617). It binds to SH3 domain of CRK and GRB2 and transduces signals from CRK to activate RAS. It plays a role in the establishment of basal endothelial barrier function and has a role in nerve growth factor-induced sustained activation of Rap1 and neurite outgrowth. Four isoforms of RAPGEF1 have been described that are produced by alternative splicing. (Ref.: Begum, Z., et al. (2018). Mol. Biol. Rep. 45(6); 1809-1819; Sasi Kumar, K., et al. (2015). Biochim. Biophys. Acta. 1853(10); 2629-2639).
特異性
Clone 3F6 is a mouse monoclonal antibody that detects Rap guanine nucleotide exchange factor 1 (RAPGEF1). It targets an epitope from the internal region.
免疫原
GST-tagged recombinant protein fragment corresponding to the Crk binding region of human RAPGEF1 (aa 268-648).
應用
Quality Control Testing
Evaluated by Western Blotting in HEK293 cells transfected with GFP-Crk-binding region (CBR).
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected RAPGEF1 in HEK293 cells transfected with GFP- Crk-binding region (CBR), but not in cells without GFP-CBR transfection.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RapGEF1 in Immunocytochemistry application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RapGEF1 in Immunoprecipitation application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).
Western Blotting Analysis: A representative lot detected RapGEF1 in Western Blotting application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819; Sriram, D., et al. (2020). Sci Rep. 10(1):18838).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HEK293 cells transfected with GFP-Crk-binding region (CBR).
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected RAPGEF1 in HEK293 cells transfected with GFP- Crk-binding region (CBR), but not in cells without GFP-CBR transfection.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RapGEF1 in Immunocytochemistry application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RapGEF1 in Immunoprecipitation application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).
Western Blotting Analysis: A representative lot detected RapGEF1 in Western Blotting application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819; Sriram, D., et al. (2020). Sci Rep. 10(1):18838).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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