生物源
rat
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
7F6, monoclonal
物種活性
human
技術
flow cytometry: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... TAPBP(6892)
一般說明
Tapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.
特異性
This antibody recognizes the Lumenal domain of Tapasin.
免疫原
Linear peptide corresponding to human Tapasin.
應用
Detect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Tapasin in 10 µg of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.
Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).
Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).
品質
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.
標靶描述
~48 kDa observed
外觀
Format: Purified
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Andreas Blees et al.
Scientific reports, 5, 17341-17341 (2015-11-28)
Salt bridges in lipid bilayers play a decisive role in the dynamic assembly and downstream signaling of the natural killer and T-cell receptors. Here, we describe the identification of an inter-subunit salt bridge in the membrane within yet another key
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