MABE433

Anti-phospho Histone H2B (Tyr37), clone 15 Antibody

clone 15, from mouse

• 别名: Histone H2B type 3-B, H2B type 12
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 15, monoclonal
• 物種活性: human, mouse
• 技術: ChIP: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_778225
• UniProt登錄號: Q8N257
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pTyr37)
• 基因資訊: human ... H2BC1(255626) ; mouse ... H2Bc1(319177)

说明

一般說明

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

免疫原

KLH-conjugated linear peptide corresponding to human Histone H2B phosphorylated at Tyr37.

應用

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in mouse MEF cells which were untreated or treated with WEE inhibitor (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in MEF-overexpressing WEE (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-phospho Histone H2B (Tyr37) antibody, clone 15 is validated for use in western blotting & ChIP for the detection of phospho Histone H2B (Tyr37).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2B (Tyr37) in 10 µg of HeLa cell lysate.

標靶描述

~17 kDa observed

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable