推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
AE25, monoclonal
物種活性
human
技術
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... EZH2(2146)
一般說明
EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis, and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.
免疫原
MBP-tagged recombinant protein corresponding to human EZH2.
應用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.
Western Blot Analysis: A 1:500 dilution from a representative lot detected EZH2 in Jurkat and HeLa cell lysates.
Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)
Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)
品質
Evaluated by Western Blot in T47D cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.
標靶描述
~80 kDa observed. Uniprot describes multiple isoforms ranging from 80-86 kDa
聯結
Replaces: 04-1047
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
T47D cell lysate
T47D cell lysate
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Xiaoning Wu et al.
The Prostate, 79(10), 1079-1089 (2019-05-20)
Enhancer of zeste 2 (EZH2) promotes prostate cancer progression. We hypothesized that increased EZH2 expression is associated with postradiotherapy metastatic disease recurrence, and may promote radioresistance. EZH2 expression was investigated using immunohistochemistry in diagnostic prostate biopsies of 113 prostate cancer
William A Scott et al.
PLoS genetics, 17(11), e1009909-e1009909 (2021-11-16)
The ATRX ATP-dependent chromatin remodelling/helicase protein associates with the DAXX histone chaperone to deposit histone H3.3 over repetitive DNA regions. Because ATRX-protein interactions impart functions, such as histone deposition, we used proximity-dependent biotinylation (BioID) to identify proximal associations for ATRX.
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