生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
192B, monoclonal
分子量
calculated mol wt 22.99 kDa
observed mol wt ~23 kDa
纯化方式
using protein G
种属反应性
human
包装
antibody small pack of 100 μg
技术
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
表位序列
Unknown
Protein ID登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... CD163(9332)
一般描述
DNA replication complex GINS protein PSF1 (UniProt: Q14691; also known as GINS complex subunit 1) is encoded by the GINS1 (also known as KIAA0186, PSF1) gene (Gene ID: 9837) in human. GINS1 is a component of the GINS (Go-Ichi-Ni-San) complex, a heterotetramer of GINS1, 2, 3, and 4, that plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex is highly conserved in mammalian species. Its each subunit is composed of two types of structural domains: an -helix rich (A) domain and a β-strand rich (B) domain. GINS1 is highly expressed in undifferentiated cells and its deficiency in mice is shown to cause embryonic lethality by day 6.5. Its levels are significantly increased in several types of cancers, including lung, breast, and prostate cancers and several cancer cell lines. Mutations in GINS1 gene are known to cause immunodeficiency 5 (IMD55) that is characterized by chronic neutropenia, natural killer cell deficiency, and recurrent viral and bacterial infections, and most patients exhibit growth retardation. (Ref.: Hsieh, H-Y., et al. (2019). Cancer Sci. 111(7); 2400-2412; Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-2095).
特异性
Clone 192B is a mouse monoclonal antibody that detects Human DNA replication complex GINS protein PSF1 (GINS1).
免疫原
Full-length recombinant human DNA replication complex GINS protein PSF1 (GINS1).
应用
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GINS1 in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GINS1 in MOLT-4 and Daudi cell lysates.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated GINS1 in Immunoprecipitation applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Immunohistochemistry Applications: A representative lot detected GINS1 in Immunohistochemistry applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Western Blotting Analysis: A representative lot detected GINS1 in Western Blotting applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Immunofluorescence Analysis: A representative lot detected GINS1 in Immunofluorescence applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GINS1 in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GINS1 in MOLT-4 and Daudi cell lysates.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated GINS1 in Immunoprecipitation applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Immunohistochemistry Applications: A representative lot detected GINS1 in Immunohistochemistry applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Western Blotting Analysis: A representative lot detected GINS1 in Western Blotting applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Immunofluorescence Analysis: A representative lot detected GINS1 in Immunofluorescence applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GINS1, clone 192B, Cat. No. MABE2033, is a mouse monoclonal antibody that detects GINS complex subunit 1 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
外形
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Recommended storage: +2°C to +8°C.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
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