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生物来源
hamster
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
Mags-21F7, monoclonal
分子量
observed mol wt ~75 kDa
纯化方式
using protein G
种属反应性
human, mouse
包装
antibody small pack of 100
技术
flow cytometry: suitable
western blot: suitable
同位素/亚型
IgG2
表位序列
Unknown
Protein ID登记号
UniProt登记号
储存温度
2-8°C
基因信息
human ... ZBTB16(7704)
特异性
Clone Mags-21F7 is an Armenian Hamster monoclonal antibody that detects Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
免疫原
A combination of peptides covering the amino, carboxyl, and hinge regions of human Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
应用
Quality Control Testing
Evaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16.
Tested Applications
Western Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).
Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16.
Tested Applications
Western Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).
Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
目标描述
Zinc finger and BTB domain-containing protein 16 (UniProt: Q05516; also known as Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF) is encoded by the ZBTB16 (also known as PLZF, ZNF145) gene (Gene ID: 7704) in human. PLZF is a member of the krueppel C2H2-type zinc-finger protein family with nine C2H2-tpe zinc-finger domains. It serves as a transcriptional repressor and this repression may be mediated through recruitment of histone deacetylases to target promoters. It affects diverse signaling pathways, including growth-regulatory and differentiation pathways and is a key regulator of myeloid development. Its expression is observed in early, but in differentiated hematopoietic cells. It is expressed in bone marrow, early myeloid cells, and peripheral blood mononuclear cells. At lower level it is also detected in the ovary, kidney, and lung tissue. PLZF is reported to be essential for development of the characteristic innate/effector functions of natural killer T (NKT) cells. It is highly expressed during the differentiation of NKT cells and is essential for the acquisition of effector/memory innate-like functions. PLZF-deficient NKT cells lose their characteristic memory phenotype, and their function is significantly impaired. These cells lose their unique ability to co-secrete IL-4 and IFN- upon ex vivo stimulation. (Ref.: Kolesnichenko, M., and Vogt, PK. (2011). Cell Cycle.10(5); 771-775; Kovalovsky, D., et al. (2010). J. Immunol. 184: 6746-6755; Melnick, AM., et al. (2000). Mol. Cell Biol. 20(6); 2075-2086).
外形
Purified Armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重悬
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommended storage: +2°C to +8°C.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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