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Merck
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Key Documents

MABC1690M

Sigma-Aldrich

Anti-RAINBOW

from mouse

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

monoclonal

物種活性(以同源性預測)

all

包裝

antibody small pack of 25 μL

技術

western blot: suitable

同型

IgG1κ

目標翻譯後修改

unmodified

一般說明

Anti-RAINBOW Antibody, Cat. No. MABC1690-25UL, validated for use in Western Blotting.
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

特異性

Anti-RAINBOW is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.

免疫原

Remazol-dyes phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

應用

Anti-RAINBOW, Cat. No. MABC1690, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Western Blotting Analysis: A representative lot detected Remazol-dye stained protein markers in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Western Blotting Analysis: A 1:250 and 1:500 dilution from a representative lot detected Remazol-dye Pre-Stained protein standards.

品質

Evaluated by Western Blotting in Pre-Stained protein standard. Western Blotting Analysis: A 1:1,000, 1:5,000, and 1:10,000 dilution of this antibody detected Remzaol-dye prestained protein standards .

外觀

Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl, pH 7.4, with 0.05% sodium azide.

其他說明

Concentration: Please refer to lot specific datasheet.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1


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