推荐产品
生物源
mouse
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
JSB-1, monoclonal
物種活性
human, hamster
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
基因資訊
human ... ABCB1(5243)
相关类别
特異性
Reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170 kDa P-gp, member of the superfamily of transmembrane transporters. JSB-1 detects P-gp overexpression in human tumor cells of all different histogenetic derivations.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
JSB-1 does not cross-react with MDR3 P-glycoprotein. JSB-1 has been shown to cross-react with Pyruvate Carboxylase (PC), an abundant Mr 130,000 mitochondrial enzyme, on both immunoblots and immunohistochemical tissue sections [Rao et al. (1995). J Histo. Cytochem. 43(12):1187-1192.] Unequivocal plasma membrane patterns of immunostaining represent true P-glycoprotein ex-presssion. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P-glycoprotein.
免疫原
Epitope: Cytoplasmic
應用
Anti-MDR1 Antibody, clone JSB-1 detects level of MDR1 & has been published & validated for use in FC, IC, IH(P) & WB.
Flow Cytometry
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen and paraffin embedded tissue
sections: 1:20.
Immunocytochemistry: acetone or air-dried preparations react well.
Western blot
Note: For cellular detection, permeabilization is required.
Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Toxicology & Drug Resistance
Toxicology & Drug Resistance
標靶描述
170 kDa
外觀
Unpurified
UnPurified mouse culture supernatant containing 0.7% BSA and 0.1% sodium azide as a preservative.
儲存和穩定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
MDR cells
MDR cells
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
T Yanagisawa et al.
British journal of cancer, 80(8), 1190-1196 (1999-06-22)
Clinical studies have suggested that both MDR1 and MRP may play a significant role in the chemosensitivity and outcome of neuroblastoma. To clarify the nature of multidrug resistance (MDR) in this tumour a series of six neuroblastoma cell lines have
Multidrug resistance P-glycoprotein monoclonal antibody JSB-1 crossreacts with pyruvate carboxylase
Rao, V V, et al
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 43, 1187-1192 (1995)
Jing Shen et al.
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 24(2), 151-159 (2008-02-20)
This study aimed to evaluate the multidrug resistance (MDR) reversal activity of quercetin (Que) in combination with hyperthermia (HT) in human myelogenous leukemia cells K562/A. The cytotoxicity of Que alone and the effect of Que and HT to doxorubicin (Dox)
Daria Brambilla et al.
International journal of cancer, 130(12), 2824-2834 (2011-07-23)
Overexpression of the mdr1 gene encoding P-glycoprotein (Pgp) exerts a major role in reducing the effectiveness of cytotoxic therapy in osteosarcoma. The interaction between actin and Pgp has been shown to be instrumental in the establishment of multidrug resistance (MDR)
Andrea Ries Thurman et al.
Journal of acquired immune deficiency syndromes (1999), 78(1), 82-92 (2018-02-10)
We describe and compare the local and systemic pharmacokinetics (PK) of tenofovir (TFV) and TFV-diphosphate (TFV-DP) in healthy premenopausal (PRE) and postmenopausal (POST) women using TFV 1% gel and correlate local PK with other mucosal end points. PRE (n =
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