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MAB3400

Sigma-Aldrich

抗波形蛋白抗体,克隆V9

clone V9, Chemicon®, from mouse

别名:

Vimentin antibody

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

V9, monoclonal

物種活性

monkey, feline, pig, bovine, rat, human, chicken, canine

應無反應活性

mouse

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

同型

IgG1

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... VIM(7431)

一般說明

波形蛋白是间充质细胞中间丝的主要亚基蛋白。据信与细胞核和质膜之间蛋白质的细胞内运输有关。波形蛋白被认为通过其作为含有脂滴的类固醇胆固醇的存储网络而参与类固醇合成的速率。与通常为阴性的癌相比,波形蛋白的免疫组织化学染色是肉瘤(神经,肌肉和成纤维细胞来源)的特征。黑色素瘤、淋巴瘤和血管肿瘤全部可对波形蛋白染色。

特異性

识别57-60 kDa的蛋白。与其他紧密相关的中间丝蛋白(包括肌间线蛋白和GFAP)没有交叉反应性。在组织切片中,该试剂对结缔组织、内皮细胞、血管平滑肌细胞和星形胶质细胞显示阳性反应。在组织培养中,典型的中间丝轮廓可见于成纤维细胞和其他细胞类型,其中含有波形蛋白

免疫原

从猪眼晶状体中纯化波形蛋白

應用

免疫印迹:
先前批次中使用了1:1000的稀释度。

免疫组织化学:
先前批次使用5-10 μg/mL;可用于采用传统高温、柠檬酸盐缓冲液处理的福尔马林固定石蜡包埋的组织。长时间福尔马林固定可降低抗体反应性。

免疫细胞化学:
5-10 μg/mL 先前批次用于 IC。

最佳工作稀释度必须由最终用户确定。
抗波形蛋白抗体,克隆V9是一种抗波形蛋白的抗体,用于IC、IH、IH(P)&WB。

品質

已通过蛋白质印迹对HeLa裂解物进行了评估。

蛋白质印迹:
该抗体已以1:1000的稀释度在10 μg HeLa裂解物中检测到波形蛋白。

標靶描述

57-60kda

聯結

替代品:MAB3410

外觀

形式:纯化
纯化的小鼠单克隆IgG1,溶于0.02 M磷酸盐缓冲液、0.25 M NaCl和0.1%叠氮化钠中。

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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实验方案

How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies

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