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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
4F5, monoclonal
物種活性
rat, rabbit
製造商／商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
rabbit ... Slc9A3(100009430)
rat ... Slc9A3(24784)
特異性
Na+/H+ exchanger, isoform NHE3.
免疫原
Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.
應用
Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
聯結
Replaces: MAB3134
外觀
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Yanling Yan et al.
Molecular and cellular biochemistry, 367(1-2), 175-183 (2012-05-24)
We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase α1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the α1 subunit
Xiaochun Li et al.
Cells, 12(11) (2023-06-10)
The current prevailing paradigm in the renin-angiotensin system dictates that most, if not all, biological, physiological, and pathological responses to its most potent peptide, angiotensin II (Ang II), are mediated by extracellular Ang II activating its cell surface receptors. Whether
Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells.
Li, XC; Hopfer, U; Zhuo, JL
American Journal of Physiology: Renal Physiology null
Elevated BSC-1 and ROMK expression in Dahl salt-sensitive rat kidneys.
Hoagland, KM; Flasch, AK; Dahly-Vernon, AJ; dos Santos, EA; Knepper, MA; Roman, RJ
Hypertension null
Brandon A Kemp et al.
Journal of the American Heart Association, 8(9), e012016-e012016 (2019-05-02)
Background Previous studies demonstrated that angiotensin (Ang) III , not Ang II , is the predominant endogenous agonist for Ang type-2 receptor ( AT 2R)-induced natriuresis in normal rats, and that hypertensive 12-week-old spontaneously hypertensive rats ( SHR ) lack
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