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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
SM1/1, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
同型
IgG2a
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... FAS(355)
特異性
Specifically recognizes Fas [CD95/APO-1]
應用
Flow cytometry: 1-10 μg/mL, with incubation for 30 minutes at 4°C. Daudi or L929 cells may be used as negative controls.
Induction of apoptosis with SM1/1:
SM1/1 induces apoptosis best when used together with a 10 molar excess of crosslinking anti-mouse IgG. The amount of antibody needed will vary depending upon the cell line used and the age of the cell and their growing conditions; best results are achieved when cells are less than 70% confluent and relatively young passage numbers, and note not all cells what express CD95 can be induced with SM1/1, the reasons why are unclear.
On Jurkat cells 100-500ng/mL of SM1/1 in the presence of 10X excess of goat anti-mouse IgG will produce ~50% kill as measured by MTT assay; without crosslinking, little or no killing will be observed.
On SKw6.4 cells 100-500ng/mL of SM1/1 in the presence of 10X excess goat anti-mouse IgG will produce ~50% kill as measured by MTT; without crosslinking ~20% or less of the cells will be induced.
On L/F15 cells 100-500ng/mL of SM1/1 with or without crosslinking IgG ~ 50% of the cells will be induced to undergo apoptosis as measured by MTT assay.
In all cases it is important to remove excess SM1/1 before adding the secondary crosslinking antibody;
Antibody additions can be either at 4C, or 37C; at 37C primary antibody should be incubated no longer than two hours prior to secondary crosslinker addition. Crosslinking antibody is typically incubated overnight, although shorter times may yield acceptable results.
As controls, cells should be incubated with an unrelated irrelevant isotype matched IgG2a antibody or with the crosslinking antibody alone.
SM1/1 monoclonal characterization is first reported in Trauth, BC et al Science 1989 245:301-5.
Optimal working dilutions must be determined by end user.
Induction of apoptosis with SM1/1:
SM1/1 induces apoptosis best when used together with a 10 molar excess of crosslinking anti-mouse IgG. The amount of antibody needed will vary depending upon the cell line used and the age of the cell and their growing conditions; best results are achieved when cells are less than 70% confluent and relatively young passage numbers, and note not all cells what express CD95 can be induced with SM1/1, the reasons why are unclear.
On Jurkat cells 100-500ng/mL of SM1/1 in the presence of 10X excess of goat anti-mouse IgG will produce ~50% kill as measured by MTT assay; without crosslinking, little or no killing will be observed.
On SKw6.4 cells 100-500ng/mL of SM1/1 in the presence of 10X excess goat anti-mouse IgG will produce ~50% kill as measured by MTT; without crosslinking ~20% or less of the cells will be induced.
On L/F15 cells 100-500ng/mL of SM1/1 with or without crosslinking IgG ~ 50% of the cells will be induced to undergo apoptosis as measured by MTT assay.
In all cases it is important to remove excess SM1/1 before adding the secondary crosslinking antibody;
Antibody additions can be either at 4C, or 37C; at 37C primary antibody should be incubated no longer than two hours prior to secondary crosslinker addition. Crosslinking antibody is typically incubated overnight, although shorter times may yield acceptable results.
As controls, cells should be incubated with an unrelated irrelevant isotype matched IgG2a antibody or with the crosslinking antibody alone.
SM1/1 monoclonal characterization is first reported in Trauth, BC et al Science 1989 245:301-5.
Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Fas Antibody, clone SM1/1 is validated for use in FC, FUNC for the detection of Fas.
聯結
Replaces: CBL527B
外觀
Format: Purified
Purified IgG provided in PBS, no preservatives.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots. After opening, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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