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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
ASC-8, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
同型
IgG1κ
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ITGB4(3691)
特異性
Human beta4 integrin (CD104)
免疫原
Human squamous cell carcinoma cells (SCC9)
應用
Immunoprecipitation: A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).
Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).
Working dilutions must be determined by end user.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).
Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).
Working dilutions must be determined by end user.
Anti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
品質
Flow Cytometry Analysis:
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.
聯結
Replaces: 05-283
外觀
Format: Purified
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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