生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
5D4E11B6, monoclonal
分子量
calculated mol wt 105.36 kDa
observed mol wt ~58 kDa
物種活性
mouse, human
物種活性（以同源性預測）
rat
包裝
antibody small pack of 100 μg
技術
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
同型
IgG2κ
運輸包裝
dry ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
特異性
Clone 5D4E11B6 is a mouse monoclonal antibody that detects A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS-1).
免疫原
Myc-His-tagged full length recombinant human ADAMTS-1.
應用
Quality Control TestingEvaluated by Western Blotting in HEK293 cell lysate.Western Blotting Analysis: A 1:125 dilution of this antibody detected ADAMTS-1 in HEK293 cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:125 dilution from a representative lot detected ADAMTS-1 in lysates from A431 cells and E16 mouse embryonic brain tissue.Western Blotting Analysis: A representative lot detected ADAMTS-1 in Western Blotting applications (Meng, T.G., et. al. (2017). Cell Death Dis. 8(6):e2846; Luque, A., et. al. (2003). J Biol. Chem. 278 (26); 23656-65).Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ADAMTS-1 in HeLa cells.ELISA Analysis: A representative lot detected ADAMTS-1 in ELISA applications (Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).Immunofluorescence Analysis: A representative lot detected ADAMTS-1 in Immunofluorescence applications (Silva, S.V., et. al. (2016). PLoS One. 11(10):e0165061; Luque, A., et. al. (2003). J Biol Chem. 278(26); 23656-65).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-ADAMTS-1, clone 5D4E11B6, Cat. No. MAB1810-I, is a mouse monoclonal antibody that detects ADAMTS-1 and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Western Blotting.
標靶描述
A disintegrin and metalloproteinase with thrombospondin motifs 1 (UniProt: Q9UHI8; also known as ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1) is encoded by the ADAMTS1 (also known as KIAA1346, METH1) gene (Gene ID: 9510) in human. ADAM-TS1 is a zinc-binding protein that is synthesized with a signal peptide (aa 1-49) and a propeptide (aa 50-252) that are subsequently cleaved by furin endopeptidase to generate the mature form (aa 253-967). It has a conserved cysteine in the cysteine-switch motif that binds the catalytic zinc ion and inhibits the enzyme. The dissociation of cysteine from the zinc ion upon the activation-peptide release activates the enzyme. It also contains spacer domain (aa 725-849) and the TSO-1 domain that are important for a tight interaction with the extracellular matrix. ADAM-TS1 cleaves aggrecan, a cartilage proteoglycan at Glu1938-Leu1939 site within the chondroitin sulfate attachment domain and is involved in its turnover. ADAMTS1 is implicated in vascularization and development and displays anti- and pro-tumorigenic activities. ADAMTS1 knockout mice exhibit reduced body weight, kidney malformation, and impaired female fertility. (Ref.: Rodriguez-Baena, FJ., et al. (2018). Sci. Rep. 8: Article 13103).
外觀
Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at +2°C to +8°C from date of receipt.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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