一般說明
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
P2Y1 is a GPCR that binds to ADP and ATP to activate Gq. A wide variety of cells and tissues express P2Y1. Vascular endothelium and smooth muscle express P2Y1, and mediate vascular tone both in the resting state and in thrombosis, when platelet-derived ATP and ADP are present at high concentrations. In addition, activation of P2Y1 expressed on platelets leads to ADP-induced shape changes and aggregation (Ralevic and Burnstock, 1998). Millipore′s cloned human P2Y1-expressing cell line is made in the 1321N1 host, which supports high levels of recombinant P2Y1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at P2Y1.
P2Y1 is a GPCR that binds to ADP and ATP to activate Gq. A wide variety of cells and tissues express P2Y1. Vascular endothelium and smooth muscle express P2Y1, and mediate vascular tone both in the resting state and in thrombosis, when platelet-derived ATP and ADP are present at high concentrations. In addition, activation of P2Y1 expressed on platelets leads to ADP-induced shape changes and aggregation (Ralevic and Burnstock, 1998). Millipore′s cloned human P2Y1-expressing cell line is made in the 1321N1 host, which supports high levels of recombinant P2Y1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at P2Y1.
細胞系描述
- GPCR Cell Lines
- Host cells: 1321N1
應用
Human P2Y1 GPCR frozen cells for Calcium Flux FLIPR Assays.
生化／生理作用
- GPCR Class: A
- Protein Target: P2Y1
- Target Sub-Family: P2Y/ Purinergic (metabotropic)
成分
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
免責聲明
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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