生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
P1B5, monoclonal
形狀
lyophilized
不包含
preservative
物種活性
human
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG1
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ITGA3(3675)
一般說明
Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.
Recognizes the α3-subunit of the α3/β1 integrin complex protein in human mononuclear cells.
This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.
免疫原
Human
human mononuclear cells
應用
Cell Inhibition Assays (1-5 µg/ml, see application references)
Flow Cytometry (1-5 µg/ml)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
Flow Cytometry (1-5 µg/ml)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
警告
Toxicity: Standard Handling (A)
外觀
Lyophilized from a volatile buffer, 100 µg BSA.
重構
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
分析報告
Positive Control
A2058 cells
A2058 cells
其他說明
Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.
Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Jia Jia et al.
Science advances, 6(28), eaaz5894-eaaz5894 (2020-09-15)
Biologically active ligands (e.g., RGDS from fibronectin) play critical roles in the development of chemically defined biomaterials. However, recent decades have shown only limited progress in discovering novel extracellular matrix-protein-derived ligands for translational applications. Through motif analysis of evolutionarily conserved
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