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APT131

Sigma-Aldrich

Caspase 3 Colorimetric Activity Assay Kit, DEVD

Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

别名:

CPP32

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.54

品質等級

物種活性(以同源性預測)

mammals

製造商/商標名

Chemicon®

技術

activity assay: suitable

UniProt登錄號

檢測方法

colorimetric

運輸包裝

dry ice

基因資訊

human ... CASP3(836)

一般說明

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.

應用

Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Research Category
Apoptosis & Cancer

成分

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution

Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

儲存和穩定性

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids


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