ABS1651

Anti-Glutathione S-Transferase A1/A2 Antibody

serum, from rabbit

• 别名: Glutathione S-transferase A1, Glutathione S-Transferase A1/A2, GST class-alpha member 1, Glutathione S-transferase Ya, Glutathione S-transferase Ya1, Glutathione S-transferase A1, N-terminally processed, Glutathione S-transferase A2, GST class-alpha memb
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse
• 物種活性（以同源性預測）: rat
• 技術: western blot: suitable
• NCBI登錄號: NP_032207; NP_032208
• UniProt登錄號: P10648; P13745
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Gsta1(14857)

说明

一般說明

Glutathione S-transferase (GST) A1 (EC:2.5.1.18; UniProt P13745; GST Ya, GST Ya1, GST A1, GST class-alpha member 1) and GST A2 (UniProt P10648; GT41A, GST Ya2, GST A2, GST class-alpha member 2) are encoded by the Gsta1 gene (Gene ID 14857) and the Gsta2 gene (Gene ID 14858), respectively, in murine species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist in either as homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24 500), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The “Y” designation refers to the “Y” fraction (as apposed to the “X and Z” fractions) they were found in during purification by Sephadex G75 chromatography. Murine Ya1 and Ya2 are different by only 11 out of their sequences (223 a.a./A1 or 223 a.a./A2). In addition, GST Ya1/Ya2 sequences are higly conserved among murine and rat species (>85% identity), while less sequence homology is found when comparing to the corresponding human proteins. Rat GST YaYa dimer was historically referred to as "Ligandin" or GST L, and old nomenclature also referred to human alpha classe GST A1-1, A1-2 and A2-2 as, respectively, B1B1, B1B2 and B2B2, where B stands for basic.

特異性

This antiserum cannot distinguish between Glutathione S-transferase A1 and A2, which share more than 95% sequence homology (UniProt P13745 & P10648).

免疫原

Purified mouse liver Glutathione S-Transferase A1/A2.

應用

Anti-Glutathione S-Transferase A1/A2 Antibody is an antibody against Glutathione S-Transferase A1/A2 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Glutathione S-Transferase A1/A2 in 10 µg of mouse testis and small intestine tissue lysate.
Western Blotting Analysis: A representative lot detected upregulated Glutathione S-transferase A1 & A2 (GSTA1/A2) in liver cytosolic preparations from mice on high-fat diet (HF) when compared with liver samples from mice on regular diet (Meakin, P.J., et al. (2014). Mol. Cell. Biol. 34(17):3305-3320).
Western Blotting Analysis: A representative lot detected anticarcinogen-induced Glutathione S-transferase A1 & A2 (GSTA1/A2) expression in liver cytosolic preparations from C57BL/6 mice received intraperitoneal beta-naphthoflavone (BNF) injection, while such induction was not observed in mouse strain DBA/2 without functional aryl hydrocarbon (Ah) receptor (McLellan, L.I., et al. (1991). Biochem. J. 276(Pt 2):461-469).
Western Blotting Analysis: A representative lot detected antioxidant-induced Glutathione S-transferase A1 & A2 (GSTA1/A2) expression in liver cytosolic preparations from mice fed with dietary 2(3)-t-butyl-4-hydroxyanisole (BHA) supplementation when compared with liver samples from mice without BHA supplementation (McLellan, L.I., and Hayes, J.D. (1989). Biochem. J. 263(2):393-402).

品質

Evaluated by Western Blotting in mouse stomach tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Glutathione S-Transferase A1/A2 in 10 µg of mouse stomach tissue lysate.

標靶描述

~25 kDa observed

外觀

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable