跳转至内容
Merck
所有图片(3)

文件

ABS1004

Sigma-Aldrich

抗磷酸NKCC1抗体(Thr212/Thr217)

serum, from rabbit

别名:

phospho-NKCC1 Thr212/Thr217, Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

human

技術

immunofluorescence: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

phosphorylation (pThr212/pThr217)

基因資訊

human ... SLC12A2(6558)

一般說明

Na-K-Cl共转运蛋白1(NKCC1)是由SLC12A2基因编码的膜蛋白,在细胞钠/钾/氯化物转运和体内平衡中起作用。它广泛分布于全身并发挥重要作用,特别是在分泌液体的腺体中。NKCC1的细胞内N末端结构域中Thr212/Thr217的磷酸化与NKCC1激活相关。

特異性

特定于磷酸化NKCC1的成熟形式。

免疫原

对应于人磷酸-NKCC1 Thr212/Thr217的线性肽。

應用

抗磷酸NKCC1 Thr212/Thr217是用于蛋白质印迹,免疫荧光的针对磷酸NKCC1的抗体。
研究子类别
信号神经科学
研究类别
信号传导
蛋白质印迹分析: 代表性批次在GMB43和GBM5细胞裂解物中检测到磷酸NKCC1 Thr212/Thr217(Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012)JBC.1-31)。
免疫荧光分析: 代表性批次在GBM43人组织中检测到磷酸NKCC1 Thr212/Thr217(Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012)JBC.1-31)。

品質

通过蛋白质印迹对GMB43细胞裂解液进行了评估。

蛋白质印迹分析: 该抗体的1:1,000的稀释液在10 µg GMB43细胞裂解物中检测到磷酸-NKCC1 Thr212/Thr217。

標靶描述

观察值〜250 kDa。NKCC1可能是NKCC1的成熟形式的二聚体,在不同的细胞中被不同的糖基化,从而导致某些裂解物中的条带未表征。

外觀

含 0.05% 叠氮化钠的兔多克隆血清。
未纯化

儲存和穩定性

自收到之日起在-20°C可稳定保存1年。
使用建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融循环,以免损坏 IgG 和影响产品性能。

其他說明

浓度:请参考特定批次的数据表。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Not finding the right product?  

Try our 产品选型工具.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Zhaoqi Zhang et al.
Journal of neuroinflammation, 19(1), 163-163 (2022-06-22)
Hydrocephalus is a severe complication of intracerebral hemorrhage with ventricular extension (ICH-IVH) and causes cerebrospinal fluid (CSF) accumulation. The choroid plexus epithelium plays an important role in CSF secretion and constitutes the blood-CSF barrier within the brain-immune system interface. Although
Chong Jiang et al.
The Journal of biological chemistry, 292(8), 3137-3145 (2017-01-15)
The ubiquitin ligase Nedd4-like (Nedd4L, or Nedd4-2) binds to and regulates stability of the epithelial Na+ channel (ENaC) in salt-absorbing epithelia in the kidney, lung, and other tissues. Its role in the distal colon, which also absorbs salt and fluid
Shamma S Rahman et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(12), 5217-5233 (2017-08-06)
The Eps15-homology domain-containing (EHD) protein family comprises 4 members that regulate endocytic recycling. Although the kidney expresses all 4 EHD proteins, their physiologic roles are largely unknown. This study focused on EHD4, which we found to be expressed differentially across
Jan Kopecky et al.
Scientific reports, 12(1), 4609-4609 (2022-03-19)
Glioblastoma has remained the deadliest primary brain tumor while its current therapy offers only modest survival prolongation. Immunotherapy has failed to record notable benefits in routine glioblastoma treatment. Conventionally, immunotherapy relies on T cells as tumor-killing agents; however, T cells
Qiguang Wang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11(17), e2306622-e2306622 (2024-02-14)
Hydrocephalus is one of the most common brain disorders and a life-long incurable condition. An empirical "one-size-fits-all" approach of cerebrospinal fluid (CSF) shunting remains the mainstay of hydrocephalus treatment and effective pharmacotherapy options are currently lacking. Macrophage-mediated ChP inflammation and

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门