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ABN302

Sigma-Aldrich

Anti-Complex I-75kD Antibody

from rabbit, purified by affinity chromatography

别名:

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, Complex I-75kD, CI-75kD

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, rat, human

物種活性(以同源性預測)

bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... NDUFS1(4719)

一般說明

Complex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Complex I-75kD.

應用

Anti-Complex I-75kD, Cat. No. ABN302, is a rabbit polyclonal antibody that detects Complex I-75kD and is tested for use in Western Blotting and Immunohistochemistry.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Tested Applications

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Complex I-75kD in rat brain, human brain, and human heart tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Complex I-75kD in human kidney and human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

品質

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Complex I-75kD in mouse brain tissue lysates.

標靶描述

~75 kDa observed

外觀

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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