生物源
rabbit
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, mouse
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
human ... TMEM259(91304)
mouse ... Tmem259(216157)
一般說明
Membralin (UniProt: Q8CIV2; also known as Transmembrane protein 259) is encoded by the Tmem259 (also known as ORF61) gene (Gene ID: 216157) in murine species. Membralin is a highly conserved protein with several transmembrane regions. However, the sequence of membralin does not show any closely related genes. It has multiple glycosylation sites and cytosolic regions at both the N-terminal ends and C-terminal ends. It is expressed in multiple tissues, including brain and spinal cord. However, it is not found in heart, skeletal muscle, and spleen. In humans its membralin expression is reported in certain cancers, including the ovarian cancers. Membralin is reported to have a role in the ER-associated protein degradation (ERAD) pathway and is required for clearance of misfolded proteins in the endoplasmic reticulum (ER). It is also required for survival of motor neurons. Membralin mutant mice display increased basal ER stress and severe and early-onset motor neuron disease and die by postnatal day 5 or 6. (Ref.: Chen YC et al. (2005). Biochimica Biophysica Acta 1730(2): 96-102.)
特異性
This polyclonal antibody specifically detects membralin in HEK293 cells overexpressing membralin.
免疫原
A Myc-conjugated linear peptide from the N-terminal region of mouse membralin.
Epitope: N-terminus
應用
Anti-Membralin Antibody, Cat. No. ABN1661, is a highly specific rabbit polyclonal antibody that targets Membralin and has been tested in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Membralin in mouse neural progenitor cells. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Immunohistochemistry Analysis: 5.0 µg/mL from a representative lot detected Membralin in mouse spinal cord tissues. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Membralin in mouse neural progenitor cells. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Immunohistochemistry Analysis: 5.0 µg/mL from a representative lot detected Membralin in mouse spinal cord tissues. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).
Research Category
Neuroscience
Neuroscience
品質
Evaluated by Western Blotting in HEK293 cells overexpressing membralin.
Western Blotting Analysis: 1 µg/mL of this antibody detected Membralin in 4 µL of lysate from HEK293 cells transfected with mouse-myc-membralin and overexpressing Membralin.
Western Blotting Analysis: 1 µg/mL of this antibody detected Membralin in 4 µL of lysate from HEK293 cells transfected with mouse-myc-membralin and overexpressing Membralin.
標靶描述
~68 kDa observed; 63.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein A + Protein G
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
Bing Zhu et al.
Nature communications, 8(1), 1472-1472 (2017-11-15)
Endoplasmic-reticulum-associated degradation (ERAD) is an important protein quality control system which maintains protein homeostasis. Constituents of the ERAD complex and its role in neurodegeneration are not yet fully understood. Here, using proteomic and FRET analyses, we demonstrate that the ER
Lu-Lin Jiang et al.
The Journal of clinical investigation, 129(8), 3103-3120 (2019-05-22)
Mechanisms underlying motor neuron degeneration in amyotrophic lateral sclerosis (ALS) are yet unclear. Specific deletion of the ER-component membralin in astrocytes manifested postnatal motor defects and lethality in mice, causing the accumulation of extracellular glutamate through reducing the glutamate transporter
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