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生物源
rabbit
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
human
物種活性（以同源性預測）
rhesus macaque (based on 100% sequence homology)
技術
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
phosphorylation (pSer386)
基因資訊
human ... IRF3(3661)
一般說明
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.
免疫原
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human IRF3 (ser386) near the C-terminus.
應用
Detect IRF-3 using this rabbit polyclonal antibody, Anti-phospho IRF3 (ser386) Antibody validated for use in western blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
品質
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected detected IRF3 (ser386) in 10 µg of Calyculin A treated MCF7 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected detected IRF3 (ser386) in 10 µg of Calyculin A treated MCF7 cell lysate.
標靶描述
~50 kDa observed
外觀
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Shamara E Davis et al.
Scientific reports, 9(1), 15485-15485 (2019-10-31)
APOL1 alleles G1 and G2 are associated with faster progression to lupus nephritis (LN)-associated end-stage renal disease (LN-ESRD) in African Americans. Increased levels of type I interferons (IFNs) and nucleosome-associated double-stranded DNA (dsDNA) fragments (nsDNA) are the hallmark of this
Ragunath Singaravelu et al.
The Journal of biological chemistry, 294(51), 19785-19794 (2019-11-07)
Upon immune recognition of viruses, the mammalian innate immune response activates a complex signal transduction network to combat infection. This activation requires phosphorylation of key transcription factors regulating IFN production and signaling, including IFN regulatory factor 3 (IRF3) and STAT1.
Samira Khiar et al.
Scientific reports, 7(1), 2561-2561 (2017-06-02)
The type I interferon response plays a pivotal role in host defense against infectious agents and tumors, and promising therapeutic approaches rely on small molecules designed to boost this system. To identify such compounds, we developed a high-throughput screening assay
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