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Merck
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ABE419

Sigma-Aldrich

Anti-Histone H3.3 Antibody, K27M mutant

from rabbit, purified by affinity chromatography

别名:

Histone H3.1, Histone H3.3

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, human

技術

ChIP: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

mutation (Lys27Met)

基因資訊

human ... H3F3B(3021)

一般說明

Histone H3.3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3.3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.

特異性

This antibody recognizes Histone H3.3 with K27M mutation.

免疫原

Epitope: Histsone H3 sequence surrounding K27M mutation
KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3.3 with K27M mutation.

應用

Anti-Histone H3.3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot detected histone H3.30 K27M mutant in diffuse intrinsic pontice Glioma tissue lysates expressing Histone H3.3 K27M mutant. (Lewis, P. W., et al. (2013). Science. 340(6134):857-861.

Chromatin Immunoprecipitation: A representative lot co-precipitated chromatin fragments containing the promoter regions of ACTA and CCT8 genes from HEK293T transfectants expressing FLAG-HA-tagged histone H3.3 with K27M mutation (Peter W. Lewis and David Allis, Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY).

品質

Evaluated by Western Blotting in MEF (H3.3) and MEF (H3.3 K27M-HA-Flag) transfected cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected FLAG-HA-tagged K27M mutant, but not wildtype, histone H3.3 in 3x10E5 cell equivalent of lysate from MEF transfectants.

標靶描述

~17 kDa observed

外觀

Immunogen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Mélanie Pagès et al.
Brain pathology (Zurich, Switzerland), 28(1), 103-111 (2016-12-17)
Ganglioglioma (GG) is a grade I tumor characterized by alterations in the MAPK pathway, including BRAF V600E mutation. Recently, diffuse midline glioma with an H3 K27M mutation was added to the WHO 2016 classification as a new grade IV entity.
Hamid Nikbakht et al.
Nature communications, 7, 11185-11185 (2016-04-07)
Diffuse Intrinsic Pontine Gliomas (DIPGs) are deadly paediatric brain tumours where needle biopsies help guide diagnosis and targeted therapies. To address spatial heterogeneity, here we analyse 134 specimens from various neuroanatomical structures of whole autopsy brains from nine DIPG patients.
Thomas J Stone et al.
Acta neuropathologica, 135(1), 115-129 (2017-10-24)
Glioneuronal tumours are an important cause of treatment-resistant epilepsy. Subtypes of tumour are often poorly discriminated by histological features and may be difficult to diagnose due to a lack of robust diagnostic tools. This is illustrated by marked variability in
Fausto J Rodriguez et al.
Brain pathology (Zurich, Switzerland), 29(1), 126-140 (2018-09-08)
Anaplasia may be identified in a subset of tumors with a presumed pilocytic astrocytoma (PA) component or piloid features, which may be associated with aggressive behavior, but the biologic basis of this change remains unclear. Fifty-seven resections from 36 patients
Sriram Venneti et al.
Acta neuropathologica, 128(5), 743-753 (2014-09-10)
Pediatric glioblastomas (GBM) are highly aggressive and lethal tumors. Recent sequencing studies have shown that ~30 % of pediatric GBM and ~80 % of diffuse intrinsic pontine gliomas show K27M mutations in the H3F3A gene, a variant encoding histone H3.3. H3F3A K27M

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