ABE1961

Anti-Borealin

serum, from rabbit

• 别名: Cell division cycle-associated protein 8, Dasra-B, hDasra-B, Pluripotent embryonic stem cell-related gene 3 protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 技術: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_060571
• UniProt登錄號: Q53HL2
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CDCA8(55143)

说明

一般說明

Borealin (UniProt: Q53HL2; also known as Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein) is encoded by the CDCA8 (also known as PESCRG3) gene (Gene ID: 55143) in human. Borealin is a novel 31-kDa chromosomal passenger protein that is conserved among all vertebrates. It is a member of the chromosomal passenger holocomplex along with Aurora B, INCENP, and survivin. This complex play a vital role at the centromere to ensure proper chromosome alignment and segregation. Borealin is required for stability of the bipolar mitotic spindle and for kinetochore error correction. Borealin levels are reported to increase during G2/M phase and are reduced following exit from the M phase of cell cycle. Depletion of Borealin by RNAi is shown to retard mitotic progression and cause spindle T misattachment and abnormalities. Borealin-depleted cells are shown to align their chromosomes to a near normal metaphase plate, but they abruptly undergo multipolar anaphases.

特異性

This polyclonal antibody detects Borealin in nocodozole treated HeLa cells.

免疫原

A His-tagged full length human recombinant Borealin.

應用

Anti-Borealin Antibody, Cat. No. ABE1961, is a highly specific rabbit polyclonal antibody that targets Borealin and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

Western Blotting Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

Immunoprecipitation Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).

品質

Evaluated by Western Blotting in Hela cell lysate treated with Nocodozole.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Borealin in 10 µg of Hela cell lysate treated with Nocodozole.

標靶描述

~36 kDa observed; 31.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

其他說明

Concentration: Please refer to lot specific datasheet.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1