生物源
rabbit
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
mouse, rat
製造商／商標名
Chemicon®
技術
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... STX1A(6804)
特異性
Recognizes Syntaxin 1A and Syntaxin 1B. The epitope does not share homology with any other known proteins.
免疫原
GST fusion protein corresponding to a cytoplasmic part of rat Syntaxin 1A (Accession number P32851) (Bennet et al. 1992; Inoue & Akagawa 1992).
應用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Syntaxin 1 Antibody is validated for use in WB, IH for the detection of Syntaxin 1.
Western blot: 1:700-1:1,250 (0.8-1.5 g/mL) using ECL on rat brain membranes.
Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
外觀
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
儲存和穩定性
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
分析報告
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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