推荐产品
製造商/商標名
Novagen®
儲存條件
OK to freeze
運輸包裝
wet ice
一般說明
BugBuster®Ni-NTA HIS-BIND®纯化试剂盒用于蛋白质纯化。Ni-NTA HIS-BIND®树脂是一种高性能的Ni2+带电琼脂糖,用于快速一步纯化含多组氨酸标签序列的蛋白质。
應用
BugBuster®Ni-NTA HIS-BIND®纯化试剂盒已用于His标记蛋白的纯化,如海藻糖-6-磷酸磷酸酶(TPP)、Ras(WT)蛋白和CsgA(主要curlin亚基)蛋白。
成分
•2 × 100 mlBugBuster 蛋白提取试剂
•10,000 U苯甲酸酯酶核酸酶,纯度> 90%
•10 mlNi-NTA His•结合树脂
•pkg/4色谱柱
•10,000 U苯甲酸酯酶核酸酶,纯度> 90%
•10 mlNi-NTA His•结合树脂
•pkg/4色谱柱
警告
毒性:多个毒性值,请参阅 MSDS(O)
其他說明
由于该运输中有害物质的性质,您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系,以获取有关这些费用的更多信息。
法律資訊
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類(WGK)
WGK 3
其他客户在看
Journal of cellular physiology, 229(9), 1245-1255 (2014-01-22)
Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis
Small (Weinheim an der Bergstrasse, Germany), 13(40) (2017-08-16)
Intermolecular interactions dominate the behavior of signal transduction in various physiological and pathological cell processes, yet assessing these interactions remains a challenging task. Here, this study reports a single-molecule force spectroscopic method that enables functional delineation of two interaction sites
PLoS pathogens, 13(4), e1006315-e1006315 (2017-04-15)
Bacterial biofilms are associated with numerous human infections. The predominant protein expressed in enteric biofilms is the amyloid curli, which forms highly immunogenic complexes with DNA. Infection with curli-expressing bacteria or systemic exposure to purified curli-DNA complexes triggers autoimmunity via
商品
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
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