推荐产品
生物源
bovine
品質等級
重組細胞
expressed in E. coli
形狀
liquid
製造商/商標名
Novagen®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
技術
targeted protein degradation: suitable
適合性
suitable for enzyme test
應用
diagnostic assay manufacturing
儲存溫度
−20°C
一般說明
Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.
成分
•50 URecombinant Enterokinase
•2 ml1X rEK Dilution/Storage Buffer
•1 ml10X rEK Cleavage Buffer
•10 µgCleavage Control Protein
•2 ml1X rEK Dilution/Storage Buffer
•1 ml10X rEK Cleavage Buffer
•10 µgCleavage Control Protein
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
單位定義
One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.
其他說明
Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.
法律資訊
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
商品
Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.
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