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Merck
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文件

69066-M

Millipore

Recombinant Enterokinase

别名:

Enterokinase Enzyme, Enterokinase Protein, Recombinant Enterokinase

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.54

生物源

bovine

品質等級

重組細胞

expressed in E. coli

形狀

liquid

製造商/商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

技術

targeted protein degradation: suitable

適合性

suitable for enzyme test

應用

diagnostic assay manufacturing

儲存溫度

−20°C

一般說明

Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.

成分

•50 URecombinant Enterokinase

•2 ml1X rEK Dilution/Storage Buffer

•1 ml10X rEK Cleavage Buffer

•10 µgCleavage Control Protein

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

單位定義

One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.

其他說明

Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.

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