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Merck
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559309

Sigma-Aldrich

PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem®

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

Drosophila, chicken, Caenorhabditis elegans, human, mouse, rat

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG

運輸包裝

wet ice

儲存溫度

−70°C

目標翻譯後修改

phosphorylation (pThr183/pTyr185)

基因資訊

human ... MAPK8(5599)

一般說明

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
  • Antibody Target Gene Symbol: MAPK8, MAPK9
  • Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
  • Entrez Gene Name: mitogen-activated protein kinase 8
  • Hu Entrez ID: 5601
  • Mu Entrez ID: 26420
  • Rat Entrez ID: 50658
  • This PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

    免疫原

    Human
    a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK

    應用

    Immunoblotting (1:1000)

    Immunohistochemistry (see comments)

    警告

    Toxicity: Standard Handling (A)

    外觀

    In Dulbecco′s PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.

    重構

    Following initial thaw, aliquot and freeze (-70°C).

    其他說明

    Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.
    Gabai, V.L., et al. 1998. FEBS Lett.438, 1.
    Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.

    法律資訊

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    儲存類別代碼

    12 - Non Combustible Liquids

    水污染物質分類(WGK)

    WGK 1

    閃點(°F)

    Not applicable

    閃點(°C)

    Not applicable


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