重組細胞
expressed in E. coli
品質等級
化驗
≥95% (SDS-PAGE)
形狀
liquid
比活性
≥5500 units/mg protein
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
異物活動
Proteases, none detected
運輸包裝
wet ice
儲存溫度
−70°C
一般說明
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
警告
Toxicity: Standard Handling (A)
單位定義
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
外觀
In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
重構
Following initial thaw, aliquot and freeze (-70°C).
其他說明
Lew, J. 2003. Biochemistry42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Milla Koistinaho et al.
Glia, 40(2), 175-183 (2002-10-16)
Although microglial cells are thought to play a beneficial role in the regeneration and plasticity of the central nervous system (CNS), recent studies have indicated that at least some molecules released by microglia may be harmful in acute brain insults
John Lew
Biochemistry, 42(4), 849-856 (2003-01-29)
Protein kinases constitute one of the largest enzyme families encoded by the human genome. Owing to their critical role in virtually all aspects of signal transduction, protein kinases have evolved stringent mechanisms for their regulation, which classically falls into two
Gary L Johnson et al.
Science (New York, N.Y.), 298(5600), 1911-1912 (2002-12-10)
Multicellular organisms have three well-characterized subfamilies of mitogen-activated protein kinases (MAPKs) that control a vast array of physiological processes. These enzymes are regulated by a characteristic phosphorelay system in which a series of three protein kinases phosphorylate and activate one
Single-Molecule Force Spectroscopy on the N2A Element of Titin: Effects of Phosphorylation and CARP.
Thomas Lanzicher et al.
Frontiers in physiology, 11, 173-173 (2020-04-08)
Titin is a large filamentous protein that forms a sarcomeric myofilament with a molecular spring region that develops force in stretched sarcomeres. The molecular spring has a complex make-up that includes the N2A element. This element largely consists of a
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