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Merck
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文件

345872

Sigma-Aldrich

Glial Derived Neurotrophic Factor, Human, Recombinant, E. coli

别名:

rhGDNF

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About This Item

分類程式碼代碼:
12352202

化驗

≥97% (SDS-PAGE)

品質等級

形狀

lyophilized

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
desiccated

溶解度

aqueous buffer: soluble (most)
water: soluble

運輸包裝

ambient

儲存溫度

−20°C

一般說明

Recombinant, human glial derived neutotrophic factor expressed in E. coli. A heterogeneously glycosylated growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. Activates receptor tyrosine kinase Ret and promotes kidney morphogenesis. Also an activator of MAP kinase. Its biological activity is destroyed by reduction of disulfide bonds.
Recombinant, human glial derived neutotrophic factor expressed in E. coli. Growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. A heterogeneously glycosylated basic protein. Its biological activity is destroyed by the reduction of disulfide bonds.

生化/生理作用

fully biologically active when compared to native GDNF by dose-dependent ³H-dopamine uptake assay

警告

Toxicity: Standard Handling (A)

準備報告

The recommended stock solution is 1 µg/100µl. For more dilute samples, add a stabilizing protein such as 0.1% BSA.

重構

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

其他說明

Vega, Q.C., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10657.
Worby, C.A., et al. 1996. J. Biol. Chem. 271, 23619.
Lin, L.F., et al. 1994. J. Neurochem. 63, 758.
Lev-Fen, H., et al. 1993. Science260, 1130.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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