219362

组织蛋白酶B，来源于人肝脏

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• 别名: 组织蛋白酶B，来源于人肝脏
• CAS号: 9047-22-7
• EC 号: 3.4.22.1 (BRENDA, IUBMB)
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 生物源: human liver
• 品質等級: 100
• 化驗: ≥95% (SDS-PAGE)
• 形狀: liquid
• 比活性: ≥10 units/mg protein
• 純化經由: affinity chromatography
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 技術: activity assay: suitable
• 適合性: suitable for molecular biology
• 應用: life science and biopharma
• 運輸包裝: wet ice
• 儲存溫度: −70°C
• 基因資訊: human ... CTSB(1508)

说明

一般說明

Research area: Cell Signaling

Native cathepsin B from human liver, purified by affinity chromatography and HPLC. The most investigated enzyme of all lysosomal cysteine proteases. Cathepsin B belongs to the papain-like family of cysteine proteases and is produced as a preproenzyme. It is a bilobal protein, and its catalytic site is situated at the interface between the two lobes.

應用

Cathepsin B, Human Liver, has been used in:

• Diagnostics: as a potent and independent prognostic marker for endometrial cancer, pancreatic adenocarcinoma, and inflammatory disease.
• Drug development: during the neovascularization process and as a potent therapeutic target for various pathologies, cancer progression, and osteoarthritis in humans.
• Pharmacology: for increasing the therapeutic index of doxorubicin by incorporating the cathepsin B cleavable spacer Phe-Lys-4-aminobenzyloxycarbonyl into an albumin-binding prodrug.
• Molecular biology: in cathepsin B activity assay.

生化／生理作用

Cathepsin B acts as both endo and exopeptidase. While as an endopeptidase it cleaves amino acids with a large hydrophobic side chain in the P2 site of the protein/peptide substrate, on the other hand as an exopeptidase it eliminates two amino acids (dipeptide) from the C terminus of a polypeptide substrate, thereby categorizing the enzyme as a peptidyl dipeptidase. It maintains homeostatic metabolic activity within cells through regular turnover of both intracellular and extracellular proteins. Additionally, it is involved in various cellular functions like regulation of pro-enzyme and pro-hormone activation, tissue remodeling, antigen processing, apoptosis, and inflammatory responses to antigens. The expression and activity of cathepsin B have been linked to several pathologies, including cardiovascular disease, cancer, Alzheimer’s, arthritis, and pancreatitis. Overexpression of cathepsin B has been observed in brain, breast, gastric, prostate, esophageal, skin, lung, ovarian, colon, and thyroid cancers and correlates positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

警告

毒性：标准处理（A）

單位定義

一个单位定义为使用100 mM Na⁺/K⁺（pH值6.0），1.33 mM EDTA和2 mM DTT作为活化缓冲液，在40°C下每分钟水解1.0 µmol Z-RR-β-萘酰胺的酶量。

外觀

在50 mM醋酸钠缓冲液、1 mM EDTA（pH 5.0）中。

準備報告

由已经认证检测显示对HBsAg以及HIV和HCV抗体呈阴性的个体组织而制备。

重構

初次解冻后，分装冻存（-70°C）。

其他說明

Hirai, K., et al. 1999.Hum.Pathol.30, 680.
Kostoulas, G., et al. 1999.FEBS Lett.455, 286.
Strojnik, T., et al. 1999.Clin. Cancer Res.5, 559.
Maquire, T.M., et al. 1998.Int. J. Biol. Markers13, 139.
Berquim, I.M., and Sloane, B.F.1996.Adv.Exp.Med. Biol.389, 281.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable