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Merck
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文件

17-294

Sigma-Aldrich

Rho激活检测试剂盒

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

品質等級

製造商/商標名

Upstate®

技術

activity assay: suitable
affinity binding assay: suitable

NCBI登錄號

UniProt登錄號

檢測方法

chemiluminescent
colorimetric
fluorometric

運輸包裝

dry ice

基因資訊

human ... RHOA(387)

一般說明

GST标签的融合蛋白,对应于小鼠Rhotekin Rho结合结构域的残基7-89,在大肠埃希菌中表达。提供与谷胱甘肽-琼脂糖结合。纯度 >90%。

應用

PP1/PP2A工具箱,用于蛋白质的选择性体外去磷酸化。

包裝

试剂盒容量:30次测定

成分

100X GTPγS,10mM(目录号20-176)

100X GDP,100mM(目录号20-177)

Mg2+裂解/洗涤缓冲液,5X(目录号20-168)

抗Rho(-A,-B,-C),克隆55(目录号05-778)

Rho检测试剂(Rhotekin RBD,琼脂糖)(目录号14-383)

品質

通过从3T3/A31细胞裂解物中沉淀GTP-Rho进行常规评估,随后使用抗Rho(05-778)通过免疫印迹分析进行检测。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形圖

CorrosionEnvironment

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

儲存類別代碼

10 - Combustible liquids


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Toshifumi Sugatani et al.
The Journal of biological chemistry, 278(7), 5001-5008 (2002-12-04)
PTEN (also known as MMAC-1 or TEP-1) is a frequently mutated tumor suppressor gene in human cancer. PTEN functions have been identified in the regulation of cell survival, growth, adhesion, migration, and invasiveness. Here, we characterize the diverse signaling networks
M P Gratacap et al.
The Journal of biological chemistry, 276(51), 47906-47913 (2001-09-19)
Platelets were used to study the activation of Rho and Rac through G-protein-coupled receptors and its regulation by cyclic nucleotides. The thromboxane A(2) (TXA(2)) mimetic rapidly activated both small GTPases independently of integrin alpha(IIb)beta(3) activation., which leads to the activation
Jeanne M Manganello et al.
The Journal of biological chemistry, 278(1), 124-130 (2002-10-26)
The present studies mapped the protein kinase A (PKA) phosphorylation site of Galpha(13) and studied the consequences of its phosphorylation. Initial experiments using purified human Galpha(13) and the PKA catalytic subunit established that PKA directly phosphorylates Galpha(13). The location of
Angeliki Louvi et al.
Development (Cambridge, England), 141(6), 1404-1415 (2014-03-07)
Loss of function of cerebral cavernous malformation 3 (CCM3) results in an autosomal dominant cerebrovascular disorder. Here, we uncover a developmental role for CCM3 in regulating neuronal migration in the neocortex. Using cell type-specific gene inactivation in mice, we show
Geraldine Pawlak et al.
The Journal of biological chemistry, 277(30), 26927-26933 (2002-05-16)
Cellular transformation by v-Src is believed to be caused by aberrant activation of signaling pathways that are normally regulated by cellular Src. Using normal rat kidney cells expressing a temperature-sensitive mutant of v-Src, we examined the role of the Raf/MEK/ERK

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