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product name
Chromolith® HighResolution RP-18 封端, L × I.D. 100 mm x 4.6 mm HPLC column
材料
PEEK column
品質等級
agency
suitable for USP L1
產品線
Chromolith®
特點
endcapped
endcapped
包裝
1 ea of
標籤範圍
15% Carbon loading
18% Carbon loading
參數
200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.
技術
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)
柱長度 × 內徑
10 cm × 4.6 mm
表面積
250 m2/g × 250 m2/g
基質
highly porous
monolithic silica
monolithic silica
基質活性組
C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase
孔徑
1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity
工作pH值範圍
2-7.5
2-7.5
分離技術
reversed phase
儲存溫度
no temp limit
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相关类别
一般說明
Chromolith®高分辨率RP-18封端柱具有直径1.15 μm的大孔隙,色谱柱效率超过140,000塔板/米。中孔为15 nm(150 Å),表面修饰为带有完整封端的十八烷基硅烷。Chromolith®高分辨率RP-18封端柱属于USP L1类。柱效率可与2.7至3μm粒径全孔隙颗粒柱相媲美。Chromolith®高分辨率RP-18封端柱非常适用于LC-MS应用。
柱长100 mm,内径4.6 mm,是所有HPLC系统的高分辨率分离的不二之选。
这种突破性硅胶整体以其独特的大孔和中孔结构组合实现了以下功能:
—在极低的柱背压下实现快速分离
—具有高容忍度基质,简化工作流程,同时省时省钱
—延长柱使用寿命,实现成本节约
—连接柱、流动梯度及使用粘度较高的移动相
Chromolith®高分辨率RP-18封端5-4.6保护小柱(3件)[1.52025]和Chromolith®保护小柱支架5 mm x 4.6 mm[1.52032]
。Chromolith®柱耦合器可通过串联多个柱(高达100,000板/柱)来显著提高Chromolith®柱的分离效率。[1.51467]
了解更多:
宣传册:在任何系统上快速完成您的分离操作。Chromolith®硅胶整体HPLC柱
所有Supelco HPLC柱,包括Chromolith®整体柱,与所有HPLC和UHPLC仪器完全兼容。
Supelco HPLC产品:所有LC仪器的最佳选择
Chromolith®高分辨率RP-18封端柱也适用于2 mm内径柱,满足UHPLC和UHPLC-MS的需要。
传单:与UHPLC性能类似 - 低压下快速且稳定 - Chromolith®高分辨率RP-18封端2 mm内径柱
應用
- A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent et al., introduces a highly sensitive LC-MS/MS method for quantifying ceftiofur in pharmaceutical preparations and milk samples. The method utilizes Chromolith® HighResolution RP-18 endcapped columns, showcasing their application in enhancing the resolution and sensitivity of chromatographic analysis in complex matrices (Levent et al., 2021).
分析報告
压力:40-70 bar
稳定性测试:通过测试
性能(蒽)
理论塔板数(N/m)(蒽): ≥ 140000
容量系数(蒽):3.9 - 4.6
拖尾因子(USP):0.90 - 1.30 %
选择性
分离因子(蒽/孕酮):1.95 - 2.35
法律資訊
儲存類別代碼
11 - Combustible Solids
商品
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
实验方案
Folic acid vs. folate differences and biological activities; implications in fortified foods and supplements for human health.
相关内容
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
小分子为离子和化合物,分子量通常低于900道尔顿。这类化合物可使用柱化学性质各异的硅胶颗粒型或整体型固定相,通过HPLC、UHPLC和LC-MS等方法有效分离和分析。
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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