14-773-M

BTK (E41K) Protein, active, 10 µg

• 分類程式碼代碼: 12352204
• eCl@ss: 32160405
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 100; 300
• 分子量: M_w 78.4 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_000061
• UniProt登錄號: P35991 (mouse); Q06187

说明

一般說明

Expressed by baculovirus in Sf21 insect cells

N-terminal His6-tagged, recombinant, full-length, human BTK

Specific mutations within the pleckstrin homology (PH) domain of BTK results in human X-linked agammaglobulinemia (XLA). The E41K substitution, identified through random mutagenesis, is a PH domain gain-of-function mutation that has been shown to be associated with increased membrane localization and tyrosine phosphorylation of BTK. Using animal models, this mutation has been shown to arrest the development of immature B cells and to act as a tumour suppressor in B-cell linker protein (BLNK/SLP-65)-deficient hosts. (Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460).

應用

Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.

Research Category
Inflammation & Immunology

生化／生理作用

• Protein Target: BTK
• Target Sub-Family:TK

包裝

Also available in 250 μg size --call for pricing and availability and reference catalog number 14-773M when ordering the 250 μg size.

品質

Routinely evaluated by phosphorylation of LKBtide.

外觀

Format: Ni²⁺/NTA-agarose

儲存和穩定性

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediat

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable