1.16884

Fractogel EMD DMAE (M)

• 别名: Fractogel^® EMD DMAE (M)
• 分類程式碼代碼: 41115711

属性

• 品質等級: 400
• ligand: diethylaminoethyl
• 描述: weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• 無菌: sterile (Caustic Stable)
• 產品線: Fractogel^®
• 形狀: resin
• 參數: 170 cm/hr flow rate; 8 bar max. pressure
• 基質活性組: methacrylate
• 平均粒徑: 40-90 μm
• 容量: 100 mg binding capacity (BSA/mg of resin)
• 轉變溫度: flash point 35 °C (calculated)
• 密度: 1.430 g/cm³
• 體積密度: 1000 kg/m³
• 應用: gene therapy; recombinant protein; vaccine development
• 分離技術: weak anion exchange
• 儲存溫度: 2-30°C

说明

一般說明

Weak anion exchange Fractogel^® resin featuring the tentacle technology, for purification of acidic and neutral proteins and peptides from multiple sources, pDNA purification, DNA, RNA, and endotoxin removal, large viruses purification, vaccine purification, blood fractionation, and more

特點和優勢

Fractogel^® EMD DMAE (M) enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

包裝

• 1.16884.0100: Fractogel^® EMD DMAE (M) Resin 100ml
• 1.16884.0010: Fractogel^® EMD DMAE (M) Resin 10ml
• 1.16884.0500: Fractogel^® EMD DMAE (M) Resin 500ml
• 1.16884.5000: Fractogel^® EMD DMAE (M) Resin 5L

Plastic bottle

分析報告

Appearance: Milky, turbid suspension,free from impurities (foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates, no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P210 - P233 - P240 - P241 - P242 - P243
• 危險分類: Flam. Liq. 3
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: 95.0 °F
• 閃點（°C）: 35 °C