07-1229

Anti-DAPK2 Antibody

from rabbit, purified by affinity chromatography

• 别名: DAP kinase, DAP- kinase-related protein, Death-associated protein kinase
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: rabbit
• 质量水平: 100
• 抗体形式: affinity purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 纯化方式: affinity chromatography
• 种属反应性: rat, mouse, human
• 技术: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同位素/亚型: IgG
• NCBI登记号: NM_014326
• UniProt登记号: Q9UIK4
• 运输: wet ice
• 靶向翻译后修饰: unmodified
• 基因信息: human ... DAPK2(23604) ; rat ... Dapk2(300799)

说明

一般描述

Death Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.

特异性

Detects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.

免疫原

Peptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.

应用

Detect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).

Immunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

质量

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.

目标描述

Detects a 42 kDa band by western blot.

联系

Replaces: AB3606

外形

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.

储存及稳定性

Stable for 6 months at 2-8° C from date of receiept.

分析说明

Control
C2C12 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable