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生物源
rabbit
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
AOW9, monoclonal
物種活性
mouse, canine, human, chicken
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... TBK1(29110)
mouse ... Tbkbp1(73174)
一般說明
TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.
特異性
Predicted to cross-react with mouse, dog, and chicken based on sequence homology.
TBK1; Does not cross-react with IKKε, IKKα or IKKβ.
免疫原
Epitope: a.a. 642-645
Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.
應用
Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.
Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
Research Category
Signaling
Apoptosis & Cancer
Signaling
Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases
Immunological Signaling
Kinases & Phosphatases
Immunological Signaling
品質
Evaluated by western blot on RIPA lysates from HEK293 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
標靶描述
85 kDa
聯結
Replaces: 05-856
外觀
Cultured supernatant containing 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
RIPA lysates from HEK293 cells.
RIPA lysates from HEK293 cells.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
Katherine A Fitzgerald et al.
Nature immunology, 4(5), 491-496 (2003-04-15)
The transcription factors interferon regulatory factor 3 (IRF3) and NF-kappaB are required for the expression of many genes involved in the innate immune response. Viral infection, or the binding of double-stranded RNA to Toll-like receptor 3, results in the coordinate
Elsa Salzer et al.
Journal of laboratory automation, 21(1), 133-142 (2015-07-15)
Chemoproteomics-based competition-binding assays allow the screening of compounds against endogenous proteins in cell or tissue extracts, but these assays are hampered by low throughput and high cost. Using compound pools rather than single compounds in a screening campaign holds the
Yetao Wang et al.
Journal of immunology (Baltimore, Md. : 1950), 189(11), 5304-5313 (2012-10-23)
Ndfip1 functions as both a recruiter and an activator of multiple HECT domain E3 ubiquitin ligases of the Nedd4 family. In this study, we demonstrate that Ndfip1 is involved in the ubiquitin-mediated degradation of mitochondrial antiviral signaling (MAVS), which is
Tao Deng et al.
Cancer research, 74(7), 2119-2130 (2014-02-04)
HER2(+) breast cancer is currently treated with chemotherapy plus anti-HER2 inhibitors. Many patients do not respond or relapse with aggressive metastatic disease. Therefore, there is an urgent need for new therapeutics that can target HER2(+) breast cancer and potentiate the
Shihao Chen et al.
Frontiers in microbiology, 11, 603131-603131 (2020-12-29)
Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, is known to cause immunosuppression and various types of cancer in chickens. Recent reports have shown that epigenetic changes in DNA and chromatin are widely implicated in the life cycle of
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