生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
7F8, monoclonal
种属反应性
mouse, human, canine
制造商/商品名称
Upstate®
技术
ELISA: suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
phosphorylation (pThr686)
基因信息
human ... ERBB2(2064)
mouse ... Erbb2(13866)
特异性
Recognizes phosphorylated erbB2 on Threonine 686
免疫原
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2
应用
Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Kinases & Phosphatases
Growth Factors & Receptors
Kinases & Phosphatases
质量
Routinely evaluated by immunoblot.
目标描述
180 kDa
外形
Subsequent thiophilic adsorption and size exclusion chromatography
Format: Purified
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
储存及稳定性
2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
X Ouyang et al.
Carcinogenesis, 19(11), 2013-2019 (1998-12-17)
Tumour cell growth may be accelerated by protein kinase C (PKC) agonists such as phorbol esters and receptor tyrosine kinases, but receptor tyrosine kinases are in turn desensitized to growth factors by PKC agonists. To clarify this apparent PKC bifunctionality
Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
Ouyang, X, et al
The Journal of Biological Chemistry, 271, 21786-21792 (1996)
Natasha M Bourgeois et al.
Frontiers in cellular and infection microbiology, 14, 1264525-1264525 (2024-04-08)
Dengue is an arboviral disease causing severe illness in over 500,000 people each year. Currently, there is no way to constrain dengue in the clinic. Host kinase regulators of dengue virus (DENV) infection have the potential to be disrupted by
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