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G005634

HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water
gradient: held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min
flow rate: 0.3 mL/min
pressure: 175 bar
column temp.: 35 °C
detector: ESI(+), m/z 100-1000
injection: 1 μL
sample: 1 μg/mL in 50:50, water: methanol

suitability

application for HPLC

application(s)

forensics and toxicology

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Benzoylecgonine
    Morphine
    Codeine
    Oxycodone
    Hydrocodone
    (±)-Deoxyephedrine hydrochloride
    Fentanyl

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

C5901

Codeine monohydrate, analytical standard

44179

Morphine sulfate salt pentahydrate, analytical standard

B-007

Benzoylecgonine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

F-002

Fentanyl solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

H-003

Hydrocodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-013

(±)-MDMA solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-022

(±)-Methamphetamine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-035

Meperidine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-017

Normeperidine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

P-011

(+)-Propoxyphene solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

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