55261-U

HybridSPE^®-Phospholipid solid phase extraction (SPE) Cartridge

Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

• Synonym(s): HybridSPE (phospholipid and protein removal) SPE cartridge tube, 1 mL
• UNSPSC Code: 41115712
• NACRES: NB.21

Properties

• product name: HybridSPE^®-Phospholipid, Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
• material: PE frit (20 μm); polypropylene hardware
• product line: HybridSPE^®
• composition: bed wt., 30 mg
• packaging: pk of 100
• technique(s): solid phase extraction (SPE): suitable
• volume: 1 mL
• matrix active group: zirconia-based phase

Description

General description

HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.

The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.

Application

• Less is more: a methodological assessment of extraction techniques for per- and polyfluoroalkyl substances (PFAS) analysis in mammalian tissues.: This study assesses various extraction methods, including HybridSPE^®-Phospholipid, for analyzing PFAS in tissues, highlighting efficiency and reproducibility (Mertens et al., 2023).


• Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: This paper describes a rapid analytical method incorporating HybridSPE^®-Phospholipid for sample preparation, demonstrating its application in environmental toxicology (Tanoue et al., 2020).

Features and Benefits

• For use in conjunction with off-line protein precipitation.

• Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
• Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
• 2-3 step generic procedure
• Minimal to no method development
• Available in 96-well and 1 mL cartridge dimensions

Legal Information

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable