It is recommended to use 0.5% non-fat dry milk in PBS-T as a dilution buffer for this primary antibody.
V9131
Anti-Vinculin Antibody
Monoclonal Anti-Vinculin antibody produced in mouse
1 of 1 reviewers received a sample product or took part in a promotion
mouse monoclonal, hVIN-1
Synonym(s):
Anti Vinculin, Vinculin Antibody, Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse
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100 μL
$770.00
0.2 ML
$996.00
About This Item
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Product Name
Monoclonal Anti-Vinculin antibody produced in mouse, clone hVIN-1, ascites fluid
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
hVIN-1, monoclonal
mol wt
antigen 116 kDa
contains
15 mM sodium azide
species reactivity
frog, chicken, mouse, canine, human, bovine, rat, turkey
enhanced validation
independent
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:400 using cultured human fibroblasts
western blot: 1:200 using extract of human fibroblasts
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
Related Categories
General description
Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.
Specificity
Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin.
Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.
Immunogen
purified human vinculin from uterus.
Application
Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
- western blotting
- immunofluorescence staining
- immunohistochemistry
Biochem/physiol Actions
Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.
Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.
Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.
Protocols
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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What is the recommended diluent for when using in western blot? E.g. 5% milk TBST, 3% BSA in PBST?
1 answer-
Helpful?
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How is V9131-.02ML packaged? Is it in a small glass container or a small plastic tube?
1 answer-
The product V9131-.02ML is packaged into a 0.5 ml polypropylene tube (PP) with a skirted base, knurls, and an assembled cap (PP).
Helpful?
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Good afternoon, regarding Vinculin antibody V9131, clone hVIN-1, I wonder if it is possible to know the epitope sequence that this Ab recognizes, as I was not able to found it. Thanks for your attention, Sincerely
1 answer-
The immunogen used to produce this antibody was purified native human vinculin. Unfortunately, the epitope sequence has not been determined.
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