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T2194

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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100 ML
$83.00
1 L
$226.00

$83.00

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100 ML
$83.00
1 L
$226.00

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

$83.00

In StockDetails

Buy in a Bundle
Request a Bulk Order

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.4

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma®hydrochloride solution has been used:
  • in the preparation of cell-lysis buffer[1][2]
  • as a component of wash buffers and RNase mix 1, used in the preparation of 5′-complete cDNAs for paired-end sequencing[3]
  • in dissolving cadmium or S2- loaded microreactors for CdS synthesis[4]

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCR1844
SLCR2945
SLCR1844
SLCR2945
SLCN2628

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Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Smith T and Phyu S
Bio-protocol, 6(22) (2016)
RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5?-Complete cDNAs
Batut P and Gingeras TR
Current Protocols in Molecular Biology, 104(1), 25B-211 (2013)
Lab-on-a-micromotor: catalytic Janus particles as mobile microreactors for tailored synthesis of nanoparticles
Pacheco M, et al.
Chemical Science, 9(42), 8056-8064 (2018)
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
Matthieu Dos Santos et al.
STAR protocols, 2(3), 100694-100694 (2021-08-13)
Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. First, it allows
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