SRP4247

MIP-1beta human

recombinant, expressed in E. coli, ≥99% (SDS-PAGE), ≥99% (HPLC)

• Synonym(s): CCL4, MIP-1- β, Macrophage inflammatory protein 1-β, Small inducible cytokine A4, T-cell activation protein 2
• MDL number: MFCD00213005
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥99% (HPLC); ≥99% (SDS-PAGE)
• form: lyophilized
• mol wt: ~7.6 kDa
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• NCBI accession no.: P13236
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CCL4(388372)

Description

General description

MIP-1β (macrophage inflammatory protein-1β), or CCL4L1 (C-C motif chemokine ligand 4 like 1), is a CC chemokine gene localized to human chromosome 17q12. It is a copy number variable gene which shares 95% sequence homology with its non-copy number variable paralogues CCL4. MIP-1β is expressed by a wide range of cells including immune cells, such as monocytes, B and T cells, fibroblasts, endothelial, and epithelial cells.

Biochem/physiol Actions

MIP-1β (macrophage inflammatory protein-1β), or CCL4L1 (C-C motif chemokine ligand 4 like 1), interacts with its major G protein-coupled receptors CCR5 and CCR8, and promotes the migration of immune cells, such as monocytes, natural killer (NK) cells, and T cells, maturation of dendritic cells (DCs), T cell and granulocyte activation, and T cell differentiation. It is an age associated inflammatory cytokine, and it shows elevated expression in the monocytes and blood of elderly individuals. MIP-1β plays a wide role in immune responses, and hence, its expression is tightly controlled during T-cell differentiation and activation.

Physical form

Lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.