SAB4200692
Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal
clone GST-2, purified from hybridoma cell culture
Synonym(s):
GST, Glutathione-S-Transferase
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GST-2, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
ELISA: suitable
dot blot: suitable
immunoblotting: 0.5 μg/mL
immunofluorescence: suitable
immunoprecipitation (IP): suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family.
Immunogen
purified recombinant GST fusion protein
Application
Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- dot blotting
- autoradiography
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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