P8982

Anti-phospho-p53 (pSer³⁹²) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• MDL number: MFCD02097164
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen 53 kDa
• species reactivity: mouse, human
• technique(s): microarray: suitable; western blot (chemiluminescent): 1:1,000 using p53 fusion proteins with CKII phosphorylation or an extract of hydroxyurea-treated MvILu cells
• UniProt accession no.: P04637
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... TP53(7157) ; mouse ... Trp53(22059)

Description

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Specificity

Does not react with non-phosphorylated p53.

Immunogen

synthetic peptide corresponding to residues around Ser³⁹² of human p53 conjugated to KLH.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Preparation Note

Purified using protein A and peptide affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable