indirect immunofluorescence: 10-20 μg/mL using NIH3T3 cells western blot: 1.5-3.0 μg/mL using rat adrenal extract (S1 fraction) and mouse testis extract (S1 fraction)
Nucleobindin 2 (NUCB2), also known as DNA binding/EF-hand/acidic protein (NEFA), p54, is encoded by the gene mapped to human chromosome 11p15. It belongs to the nucleobindin family of EF-hand Ca2+ - binding proteins. NUCB2 contains multiple functional domains, including a Leu/Ile rich region, a putative nuclear localization signal, a DNA binding domain, two Ca2+ -binding EF-hand motifs and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. NUCB2 has been shown to interact with both necdin and ARTS-1, through the EF-hand motif.
Specificity
Anti-NUCB2 (C-terminal) specifically recognizes rat and mouse NUCB2.
Application
Anti-NUCB2 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Nucleobindin 2 (NUCB2) has been implicated in various functions, including Ca2+ homeostasis, hypothalamic regulation of feeding and tumor necrosis factor (TNF)-receptor-1 (TNFR1)shedding. The NUCB2-ARTS-1 complex is essential for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been identified as a novel satiety molecule in the hypothalamus associated with melanocortin signaling in the central nervous system. NUCB2/nesfatin-1 has been shown to induce suppression of food-intake in rats.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Candidate genes in patients with autoinflammatory syndrome resembling tumor necrosis factor receptor-associated periodic syndrome without mutations in the TNFRSF1A gene
Borghini S, et al.
The Journal of Rheumatology, 38(7), 1378-1384 (2011)
Peripheral administration of nesfatin-1 reduces food intake in mice: the leptin-independent mechanism
Inducers of satiety are drug targets for weight loss to mitigate obesity-associated diseases. Nucleobindin-2 (Nucb2) is thought to be post-translationally processed into bioactive nesfatin-1 peptide, which reportedly induces satiety, causes weight loss, and thus improves insulin sensitivity. Here, we show
Modulation of nucleobindin-1 and nucleobindin-2 by caspases
Valencia CA, et al.
Febs Letters, 582(2), 286-290 (2008)
Identification of nesfatin-1 as a satiety molecule in the hypothalamus
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