N6514

Neuraminidase from Vibrio cholerae

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

• Synonym(s): Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• CAS Number: 9001-67-6
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• EC Number: 232-624-6
• MDL number: MFCD00131711
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Vibrio cholerae
• Quality Level: 200
• type: Type II
• form: buffered aqueous solution
• specific activity: 8-24 units/mg protein (Lowry, using NAN-lactose)
• foreign activity: Protease and NAN-aldolase, present
• storage temp.: 2-8°C

Description

General description

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

Application

Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered..

Preparation Note

A further purification by affinity chromatography of our Type III.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves