N2635
Nucleoside 5′-Diphosphate Kinase from bovine liver
buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)
Synonym(s):
NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK
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100 UNITS
$478.00
500 UNITS
$1,400.00
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100 UNITS
$478.00
500 UNITS
$1,400.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Recommended Products
biological source
bovine liver
Quality Level
form
buffered aqueous glycerol solution
specific activity
≥1,000 units/mg protein (biuret)
storage condition
(Tightly closed)
technique(s)
activity assay: suitable
foreign activity
lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
storage temp.
2-8°C
General description
Application
Nucleoside 5′-Diphosphate Kinase has been used:
- nucleoside diphosphate kinase (NDPK) assays[3]
- coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)[4]
- non-metastatic protein 23 (NM23) growth stimulation assay[5]
- in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells [6]
- in a study to investigate protein synthesis in rabbit reticulocytes[7]
Biochem/physiol Actions
Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs).[1] NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.[8]
Unit Definition
One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.
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enzyme
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substrate
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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